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Open Access Highly Accessed Research article

In young children, persistent wheezing is associated with bronchial bacterial infection: a retrospective analysis

Iris De Schutter1*, Alexandra Dreesman1, Oriane Soetens2, Marc De Waele3, Françoise Crokaert4, Jan Verhaegen5, Denis Piérard2 and Anne Malfroot1

Author Affiliations

1 Department of Pediatric Pulmonology, CF-Clinic and Pediatric Infectious Diseases, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium

2 Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium

3 Department of Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium

4 Department of Microbiology, CHU St. Pierre, Brussels, Belgium

5 Department of Microbiology, UZ, Gasthuisberg, Leuven, Belgium

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BMC Pediatrics 2012, 12:83  doi:10.1186/1471-2431-12-83

Published: 22 June 2012

Abstract

Background

Young children with persistent wheezing pose a diagnostic and therapeutical challenge to the pediatrician.

We aimed to evaluate bacterial bronchial infection as a possible reason for non response to conventional asthma therapy, and to identify and characterise the predominant pathogens involved.

Methods

We retrospectively analysed microbiological and cytological findings in a selected population of young wheezers with symptoms unresponsive to inhaled corticosteroid (ICS) therapy, who underwent flexible bronchoscopy with bronchoalveolar lavage (BAL). Procedural measures were taken to limit contamination risk and quantitative bacterial culture of BAL fluid (significance cut-off ≥ 104 colony-forming units/ml) was used. Modern microbiological methods were used for detection of a wide panel of pathogens and for characterisation of the bacterial isolates.

Results

33 children aged between 4 and 38 months, without structural anomalies of the conductive airways were evaluated. Significant bacterial BAL cultures were found in 48,5 % of patients. Haemophilus influenzae was isolated in 30,3 %, Streptococcus pneumoniae in 12,1 % and Moraxella catarrhalis in 12,1 %. All H. influenzae isolates were non-encapsulated strains and definitely distinguished from non-haemolytic H. haemolyticus. Respiratory viruses were detected in 21,9 % of cases with mixed bacterial-viral infection in 12,1 %. Cytology revealed a marked neutrophilic inflammation.

Conclusions

Bacterial infection of the bronchial tree is common in persistent preschool wheezers and provides a possible explanation for non response to ICS therapy. Non-typeable H. influenzae seems to be the predominant pathogen involved, followed by S. pneumoniae and M. catarrhalis.

Keywords:
Preschool wheezing; Bronchial bacterial infection; Neutrophilic inflammation; Non-Typeable Haemophilus Influenzae