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Open Access Research article

Agreement between Orbscan II, VuMAX UBM and Artemis-2 very-high frequency ultrasound scanner for measurement of anterior chamber depth

Haya Matuoq Al Farhan

Author Affiliations

Department of Optometry and Vision Sciences, College of Applied Medicine Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

BMC Ophthalmology 2014, 14:20  doi:10.1186/1471-2415-14-20

Published: 25 February 2014



The aim was to compare the anterior chamber depth (ACD) measurements taken with Orbscan II, ultrasound biomicroscopy (UBM) and the Artemis-2 VHF (very-high-frequency) ultrasound scanner in normal subjects.


In this prospective study, one eye from each of 60 normal subjects was randomly selected. Three subjects dropped out of the study because they were apprehensive about the UBM examination; their data were excluded entirely. Measurements of ACD were taken with the Orbscan II, UBM and Artemis-2 VHFUS. Results were obtained for coefficient of variance (CV) and intra-class correlation coefficient (ICC), and statistical analysis was by repeated-measures analysis of variance (ANOVA) for intra-observer repeatability. ANOVA and Bland–Altman analyses were used to determine limits of agreement (LOA) between the three instruments.


The average ACD (± standard deviation) was 3.13 ± 0.34 mm, 2.96 ± 0.27 mm and 2.87 ± 0.31 mm for the Orbscan II, UBM and Artemis-2 VHFUS, respectively. The repeatability scores were 0.015 ± 0.014%, 0.08 ± 0.09% and 0.07 ± 0.06% for the Orbscan II, UBM and Artemis-2 VHFUS, respectively. The ICC for repeatability of Orbscan II, UBM and Artemis-2 VHFUS measurements was high and equal to 0.99%. The intra-observer repeatability scores of the ACD measurement p-values using Orbscan II, UBM and Artemis-2 VHFUS were 0.12, 0.70 and 0.10, respectively. The mean difference and standard deviations for ACD measurements using Orbscan II vs UBM, Orbscan II vs Artemis-2 VHFUS and UBM vs Artemis-2 VHFUS were 0.17 ± 0.31 mm, 0.27 ± 0.34 mm and 0.10 ± 0.18 mm, respectively. LOAs were 0.78 to -0.44 mm, 0.93 to -0.39 mm and 0.45 to -0.26 mm. ANOVA revealed a statistically significant difference between the Orbscan II, UBM and Artemis-2 VHFUS (p < 0.0001).


Measurements by the three instruments show high repeatability. UBM and the Artemis-2 VHFUS can be used interchangeably, but the Orbscan II cannot be used interchangeably with UBM or the Artemis-2 VHFUS.

Anterior chamber depth; Artemis-2 VHF; Normal eyes; Orbscan II; Ultrasound biomicroscopy; Ultrasound scanner