Intravitreal injection of siRNA. A. Fluorescent micrograph of a cryostat section and B), C) whole mount retinas prepared 24 h after single intravitreal injection with 20 ng fluorescent siRNA (red) combined with Transit-TKO in low ionic environment. Note, the prominent localization of siRNA in the ganglion cells layer (GCL) of the retina. Arrows indicate the examples of cytoplasmic accumulation of siRNA near to cells nuclei. Low and highly transfected regions of the retina are marked by ovals (I, II). Cells nuclei were counterstained with Hoechst (blue). Scale bar = 20 μm.
Turchinovich et al. BMC Ophthalmology 2010 10:25 doi:10.1186/1471-2415-10-25