Table 4 |
||||||
|
HPV detection and typing of 15 HNSCC and 3 RRP patients. |
||||||
|
HPV detection method |
||||||
|
|
||||||
|
Patient |
Age |
Diagnosis |
PGMY 09/11 |
Linear Array |
GP5+/GP6+ nest |
|
|
|
||||||
|
1 |
52 |
SCC |
- |
- |
HPV 6 |
|
|
|
||||||
|
2 |
46 |
SCC |
- |
- |
HPV 6 |
|
|
|
||||||
|
3 |
49 |
SCC |
- |
- |
HPV 16, mixed |
|
|
|
||||||
|
4 |
58 |
RRP |
- |
- |
- |
|
|
|
||||||
|
5 |
68 |
SCC |
- |
HPV 16 |
HPV 16 |
|
|
|
||||||
|
6 |
31 |
RRP |
HPV 11 |
HPV 11 |
HPV 11 |
|
|
|
||||||
|
7 |
61 |
SCC |
- |
- |
- |
|
|
|
||||||
|
8 |
68 |
SCC |
- |
- |
- |
|
|
|
||||||
|
9 |
54 |
SCC |
- |
HPV 16 |
HPV 6 |
|
|
|
||||||
|
10 |
64 |
SCC |
- |
- |
HPV 6 |
|
|
|
||||||
|
11 |
72 |
SCC |
- |
- |
HPV 16 |
|
|
|
||||||
|
12 |
73 |
SCC |
- |
HPV 11 |
HPV 6 |
|
|
|
||||||
|
13 |
87 |
RRP |
- |
- |
- |
|
|
|
||||||
|
14 |
69 |
SCC |
- |
- |
- |
|
|
|
||||||
|
15 |
74 |
SCC |
- |
- |
- |
|
|
|
||||||
|
16 |
50 |
SCC |
- |
- |
HPV 40 |
|
|
|
||||||
|
17 |
78 |
SCC |
- |
- |
mixed types |
|
|
|
||||||
|
18 |
74 |
SCC |
- |
- |
- |
|
|
|
||||||
|
HPV +ve HNSCC detection |
0/15 |
3/15 |
10/15 |
|||
|
|
||||||
|
PCR detection, using the PGMY09/11 primer set was unable to detect the presence of HPV in any of the head and neck squamous cell carcinoma (SCC) samples (0/15). Improved detection was achieved using the Linear Array assay (3/15) and following a nested PCR of the PGMY reaction with the GP5+/GP6+ primer set (10/15). HPV was only detected in 1/3 of recurrent respiratory papillomatosis (RRP) samples, irrespective of the method used. Samples were scored as positive if HPV was detected using one or more of the methods employed. |
||||||
|
Winder et al. BMC Cancer 2009 9:440 doi:10.1186/1471-2407-9-440 |
||||||
