Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes
1 Department of Urology, University of Washington, Seattle WA 98195, USA
2 Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle WA 98195, USA
3 Institute for Systems Biology, Seattle WA 98103, USA
4 Department of Medicinal Chemistry, University of Washington, Seattle WA 98195, USA
5 Department of Genetics, University of Sao Paulo's Medical School at Ribeirão Preto, Brazil
6 Department of Pathology, University of Washington, Seattle WA 98195, USA
7 LEP: Department of Urology, University of Pittsburgh Medical Center, Pittsburgh, PA 15232, USA
BMC Cancer 2009, 9:317 doi:10.1186/1471-2407-9-317Published: 8 September 2009
The prostate stroma is a key mediator of epithelial differentiation and development, and potentially plays a role in the initiation and progression of prostate cancer. The tumor-associated stroma is marked by increased expression of CD90/THY1. Isolation and characterization of these stromal cells could provide valuable insight into the biology of the tumor microenvironment.
Prostate CD90+ stromal fibromuscular cells from tumor specimens were isolated by cell-sorting and analyzed by DNA microarray. Dataset analysis was used to compare gene expression between histologically normal and tumor-associated stromal cells. For comparison, stromal cells were also isolated and analyzed from the urinary bladder.
The tumor-associated stromal cells were found to have decreased expression of genes involved in smooth muscle differentiation, and those detected in prostate but not bladder. Other differential expression between the stromal cell types included that of the CXC-chemokine genes.
CD90+ prostate tumor-associated stromal cells differed from their normal counterpart in expression of multiple genes, some of which are potentially involved in organ development.