Figure 3.

Summary of Cripto-1 expression before and after lentivirus-mediated RNA interference. Real-time RT-PCR showed that the Cripto-1 mRNA in CNE-2 (a) and C666-1 cells (c) were effectively knockdown by RNAi. * p < 0.05. Protein expression were detected by western blot analysis, Cripto-1 were specifically inhibited in CNE-2/GFP⁺/Cripto-1^- (b) and C666-1/GFP⁺/Cripto-1^- cells (d). (p < 0.05, respectively).