Figure 6.

Concentrations of auraptene in rat tissues from the tumor study. The rat mammary glands, tumors and livers were weighed and homogenized. To the homogenate 80 μL of 100 μM curcumin was added as internal standard and extracted twice with ethyl acetate: acetone mixture (2:1 v/v). The extract was dried and resuspended in 1 mL 70% methanol/H₂O and analyzed by HPLC (Shimadzu, SIL-10AF) with UV (254 nm) and fluorescence detection (Shimadzu, RF-535) at excitation and emission wavelengths of 329 nm and 390 nm respectively.