Figure 6.

Effects of IBTX and siRNA on transendothelial migration of breast cancer cells. A: Representative images of breast cancer cells that adhered to monolayer of the human brain microvasculature endothelial cell line HCMEC/D3. The adherent breast cancer cells appear white in color against the background of HCMEC/D3. (See arrows). B: Breast cancer cells pretreated with IBTX or siRNA to silence the KCNMA1 gene demonstrated significantly (*P < 0.05; **P < 0.001) reduced BK_Ca channel mediated-TEM of breast cancer cells across HCMEC/D3 monolayer compared to untreated control. Data is presented as percentage migration of treated breast cancer cells compared with migration of untreated controls (100%); Bars represent the mean ± standard error of the mean obtained from three different experiments (n = 3).