Figure 4.

Overexpression of MYCNOS in the NB cell line IMR-32. (A) Transfection-efficiency was measured by GFP expression as analysed by flow cytometry, 74% of the NB cells expressed GFP 72 hours after transfection. (B) In the MYCNOS transfected IMR-32 cells, MYCNOS was 50× upregulated compared to not transfected and mock-transfected IMR-32 cells. Endogenous MYCN expression was not significantly affected. (C) Western blot showing that MYCN protein expression in MYCNOS transfected IMR-32 cells was unaffected. Lane 1 is loaded with lysate from untransfected cells, lane 2 with lysate from cells transfected with the empty vector, and lane 3 with lysate from MYCNOS transfected cells.