Table 2 |
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|
Expression profiles of selected genes and proteins from microarray analysis, RT-PCR, immunocytochemistry and western blot analysis. |
|||||||
|
GENE CELL |
N25 |
N32 |
N29 |
RN33B |
ST14A |
HIB5 |
ANALYSIS |
|
|
|||||||
|
Follistatin |
+ |
- |
+/- |
- |
+++ |
+++ |
Microarray |
|
+ |
- |
- |
- |
+++ |
++ |
RT-PCR |
|
|
+ |
+/- |
- |
- |
+++ |
+++ |
Protein exp |
|
|
|
|||||||
|
GAS6 (growth arrest specific protein 6) |
+/- |
+/- |
+/- |
+/- |
+++ |
+++ |
Microarray |
|
+/- |
+/- |
+/- |
- |
+++ |
++ |
RT-PCR |
|
|
- |
- |
- |
+/- |
++ |
++ |
Protein exp |
|
|
|
|||||||
|
TAPA1/CD81 |
+ |
missing |
++ |
+ |
+++ |
+++ |
Microarray |
|
+ |
+++ |
+ |
+/- |
+++ |
++ |
RT-PCR |
|
|
++ |
+++ |
+++ |
- |
++ |
+++ |
Protein exp |
|
|
|
|||||||
|
AXL |
missing |
missing |
missing |
missing |
missing |
missing |
Microarray |
|
++ |
+ |
++ |
+ |
+++ |
++ |
RT-PCR |
|
|
+++ |
++ |
+++ |
- |
++ |
+++ |
Protein exp |
|
|
|
|||||||
|
GRO1/CXCL1 (chemokine (C-X-C motif) ligand 1) |
+++ |
++ |
++ |
++ |
+ |
+ |
Microarray |
|
++ |
+++ |
+ |
- |
++ |
++ |
RT-PCR |
|
|
- |
+++ |
++ |
- |
- |
- |
Protein exp |
|
|
|
|||||||
|
TPT1 (tumor protein, translationally controlled 1) |
++ |
+ |
++ |
+/- |
+++ |
+++ |
Microarray |
|
+++ |
++ |
+ |
+/- |
++ |
+ |
RT-PCR |
|
|
|
|||||||
|
TRIM23/Arfd1 (tripartite motif-containing 23) |
+ |
+ |
+ |
+ |
+++ |
+++ |
Microarray |
|
+ |
+ |
+ |
++ |
+++ |
++ |
RT-PCR |
|
|
|
|||||||
|
CD82/Kai1 |
++ |
++ |
+++ |
+ |
+++ |
+++ |
Microarray |
|
+++ |
++ |
++ |
+ |
+++ |
+++ |
RT-PCR |
|
|
|
|||||||
|
Camk2 (Ca/calmodulin dependent protein kinase typeII) |
++ |
+ |
+ |
+ |
+++ |
++ |
Microarray |
|
++ |
+++ |
+ |
+ |
++ |
++ |
RT-PCR |
|
|
|
|||||||
|
Eef1a (Elongation factor 1 alpha) |
++ |
+ |
+ |
+ |
+++ |
+++ |
Microarray |
|
+++ |
+ |
+ |
+ |
++ |
++ |
RT-PCR |
|
|
|
|||||||
|
The order of the scores in the table is 1) microarray, 2) RT-PCR, 3) protein analysis. The selected and compared genes were: Growth arrest specific 6 (gas6), follistatin (fst), AXL receptor tyrosine kinase (missing on the array), translationally-controlled 1 (TPT1), Kai (CD82), CD81 (Tapa-1), ADP-ribosylation factor domain protein 1 (Arfd1), Calcium/calmodulin-dependent protein kinase II (Camk2), growth regulated protein precursor CXCL1 and Eukaryotic translation elongation factor 1 alpha 1 (Eefa1). The levels of expression are compared within each method of measurement used and expressed within each group as +++ for the strongest expression measured. ++ was set for values ranging from 45–70% of the highest score (+) for 15–44% of the highest score, (+/-) for 1–14% of the highest score and (-) for no expression detected. |
|||||||
|
Staflin et al. BMC Cancer 2009 9:206 doi:10.1186/1471-2407-9-206 |
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