Email updates

Keep up to date with the latest news and content from BMC Cancer and BioMed Central.

Open Access Research article

249 TP53 mutation has high prevalence and is correlated with larger and poorly differentiated HCC in Brazilian patients

Jeronimo A Nogueira1, Suzane K Ono-Nita1*, Marcelo E Nita1, Marcelo MT de Souza1, Eliane P do Carmo1, Evandro S Mello2, Cristovan Scapulatempo2, Denise C Paranaguá-Vezozzo1, Flair J Carrilho1 and Venancio AF Alves2

  • * Corresponding author: Suzane K Ono-Nita skon@usp.br

Author Affiliations

1 Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil

2 Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil

For all author emails, please log on.

BMC Cancer 2009, 9:204  doi:10.1186/1471-2407-9-204

Published: 26 June 2009

Abstract

Background

Ser-249 TP53 mutation (249Ser) is a molecular evidence for aflatoxin-related carcinogenesis in Hepatocellular Carcinoma (HCC) and it is frequent in some African and Asian regions, but it is unusual in Western countries. HBV has been claimed to add a synergic effect on genesis of this particular mutation with aflatoxin. The aim of this study was to investigate the frequency of 249Ser mutation in HCC from patients in Brazil.

Methods

We studied 74 HCC formalin fixed paraffin blocks samples of patients whom underwent surgical resection in Brazil. 249Ser mutation was analyzed by RFLP and DNA sequencing. HBV DNA presence was determined by Real-Time PCR.

Results

249Ser mutation was found in 21/74 (28%) samples while HBV DNA was detected in 13/74 (16%). 249Ser mutation was detected in 21/74 samples by RFLP assay, of which 14 were confirmed by 249Ser mutant-specific PCR, and 12 by nucleic acid sequencing. All HCC cases with p53-249ser mutation displayed also wild-type p53 sequences. Poorly differentiated HCC was more likely to have 249Ser mutation (OR = 2.415, 95% CI = 1.001 – 5.824, p = 0.05). The mean size of 249Ser HCC tumor was 9.4 cm versus 5.5 cm on wild type HCC (p = 0.012). HBV DNA detection was not related to 249Ser mutation.

Conclusion

Our results indicate that 249Ser mutation is a HCC important factor of carcinogenesis in Brazil and it is associated to large and poorly differentiated tumors.