Prostasin and HAI-1 are located on the apical and the basolateral side, respectively in normal tissue and some colorectal cancer tissue specimens whereas they are co-localized in other colorectal cancer tissues specimens. Tissue sections of formalin-fixed, paraffin-embedded normal and malignant tissue from colorectal cancer patients was immunohistochemically stained for prostasin (A, E, I) and HAI-1(B, F, J). Histological normal colorectal tissue from a cancer patient (upper panel, A, B, C and D) and two examples of colorectal cancer tissue are shown (middle and lower panel). Nuclei were visualized by DAPI staining (C, G, and K).
Selzer-Plon et al. BMC Cancer 2009 9:201 doi:10.1186/1471-2407-9-201