Table 2

Ectopic expression of microRNAs

Name

Vector

Vector IC

miR

miR IC

V:DCT

miR:DCT

DDCT

Fold change


19a

20.1

14.5

18.4

14.1

5.6

4.3

-1.2

2.4


491-3p

24.4

14.3

22.0

14.2

10.1

7.8

-2.4

5.1


296-3p

22.8

14.5

17.3

14.1

8.3

3.1

-5.2

36.1


532-3p

22.0

14.3

20.5

14.2

7.7

6.2

-1.5

2.9


188-3p

25.0

14.5

21.4

15.0

10.6

6.4

-4.2

17.9


579

27.3

14.5

23.3

14.1

12.9

9.2

-3.7

12.8


501-5p

23.0

14.5

19.2

14.1

8.5

5.1

-3.4

10.7


548c-3p

26.7

14.5

24.8

14.1

12.3

10.7

-1.6

3.1


219-1-3p

26.0

14.5

19.4

14.4

11.5

5.0

-6.5

92.9


519b-3p

28.6

14.3

22.9

14.2

14.3

8.7

-5.6

48.8


155

33.6

17.1

25.3

17.5

16.5

7.8

-8.7

415.9


519e

28.6

14.3

22.9

14.2

16.0

8.7

-7.3

155.0


139-3p

24.4

14.5

16.7

13.7

9.9

3.0

-6.9

118.5


203

26.2

14.5

15.3

14.1

11.7

1.2

-10.5

1436.2


142-3p

31.9

14.5

21.2

14.1

17.5

7.1

-10.3

1296.1


450b-5p

21.3

14.5

17.3

15.0

6.9

2.3

-4.5

23.2


193a-3p

24.9

14.5

19.0

14.4

10.4

4.7

-5.8

55.1


193b

24.0

14.3

20.3

14.1

9.7

6.2

-3.5

11.5


487b

26.3

14.3

17.6

14.1

12.1

3.4

-8.6

391.9


487a

28.3

14.5

19.0

15.0

13.9

4.1

-9.8

900.1


301a

25.1

14.5

22.0

15.0

10.6

7.1

-3.6

11.8


130a

25.0

14.3

16.1

14.1

10.8

2.0

-8.8

431.6


130b

20.4

14.5

17.4

14.3

5.9

3.2

-2.7

6.5


148a

21.1

14.7

16.6

14.6

6.4

2.0

-4.2

18.1


503

24.8

18.6

20.4

18.1

6.2

2.3

-3.9

14.5


152

24.2

14.5

15.5

14.1

9.7

1.4

-8.4

327.0


95

23.4

14.5

16.0

14.2

8.8

1.8

-7.0

128.3


550

21.7

14.5

16.1

14.4

7.2

1.7

-5.5

44.7


424

18.0

14.5

14.6

14.3

3.5

0.4

-3.1

8.5


16

16.8

14.3

15.6

14.2

2.5

1.4

-1.2

2.3


195

17.3

14.3

15.2

14.2

3.0

1.0

-2.1

4.2


144

30.1

14.5

24.2

14.2

15.6

10.0

-5.6

47.0


34a

27.4

15.5

19.0

14.6

11.9

4.4

-7.5

187.3


449a

25.2

14.3

15.6

14.2

10.9

1.4

-9.5

723.8


146a

34.6

14.5

25.6

14.5

20.1

11.1

-9.0

505.0


655

29.7

14.5

27.4

14.2

15.2

13.3

-1.9

3.8


374b

19.5

14.5

16.8

14.2

5.0

2.7

-2.3

5.1


520d-5p

31.8

14.5

25.8

13.7

17.3

12.1

-5.2

36.8


376c

29.3

14.5

18.7

14.1

14.9

4.6

-10.3

1296.1


23b

19.2

14.3

18.0

14.2

4.9

3.7

-1.2

2.2


199a-3p

27.6

14.5

17.5

14.1

13.1

3.4

-9.8

864.1


194

23.7

14.5

17.4

14.1

9.2

3.2

-6.0

62.4


202

31.2

14.5

18.4

13.7

16.7

4.7

-12.0

3959.2


875

23.4

14.5

15.7

13.6

8.8

2.1

-6.7

104.5


511

25.4

14.5

20.5

14.4

10.9

6.1

-4.9

29.1


Values are CTs except for fold change. IC, Internal controls, average of 5sRNA, U6 and β-actin; V, vector.

Jiang et al. BMC Cancer 2009 9:194   doi:10.1186/1471-2407-9-194

Open Data