Figure 1.

Effect of PSK on tumour cell line proliferation. B16, A549, Hela, AGS, Jurkat, B9 and Ando-2 tumour cell lines were treated or not with 50 μg/ml or 100 μg/ml of PSK for 72–96 h. Tumour cells (2.5 to 5 × 10⁴) were plated in quadruplicate in 96-well plates. The proliferation of tumour cells was determined by BrdU incorporation and absorbance measurement. All cell lines analysed showed inhibition of proliferation. Each column represents the mean of five independent experiments ± SD. P < 0.001 versus control.