Quantification of leukemic blasts by flow cytometry using admix assay. a. Dilutions of diagnostic samples admixed to PB or BM samples, from various time-points of follow-up during chemotherapy for lineage-unrelated leukaemia (e.g. B-lineage blasts into T-ALL follow-up BM and vice-versa) and analysed for MRD in R3 region using the same template as described in Materials and Methods. Correlation of the data was examined by regression analysis; line of identity is shown by (-). Diagnostic PB from B-ALL mixed with T-ALL follow-up (52 weeks of treatment, black rhombus); diagnostic BM from B-ALL mixed with T-ALL follow-up (52 weeks of treatment, black triangle); diagnostic BM from T-ALL mixed with B-ALL follow-up (90 weeks of treatment, O); diagnostic BM from T-ALL mixed with B-ALL follow-up (104 weeks of treatment, black square) b. Dilutions of the REH (B-ALL, white triangle) and MOLT-4 (T-ALL, white rhombus) cell lines were made with normal PBMC and analysed as above.
Chowdhury et al. BMC Cancer 2008 8:40 doi:10.1186/1471-2407-8-40