Additional file 3.
Normalization gene selection. The expression level of 6 housekeeping genes (HSK) was determined on 150 breast cancer tissue samples. Gene stability was evaluated using the geNorm program [Ref. ] which relies on the principle that the expression ratio of two ideal reference genes would be identical in all samples, regardless of experimental conditions or cell type. The program calculates the gene stability measure (M) which is the average pair-wise variation for a gene compared with all other tested control gene. Genes with lower M values are more stable. The results show that PPIG, SLU7, and NUP214 were the most stable housekeeping genes in this sample set.
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Tutt et al. BMC Cancer 2008 8:339 doi:10.1186/1471-2407-8-339