Electropherograms of allelic shifts. Electropherograms of shifted alleles. DNA fragment analysis of a representative set of PTP cMNRs in a MSI-H colorectal tumor (KS2966A, Sam49) and MSI-H cancer cell lines (Co115, TC71, and HCT116). Corresponding wildtype alleles (wt, lower panel) are indicated together with tumor cell-specific allelic shifts marked by asterisks (upper panel). Allele intensities were determined (peak area) and ratios (f) of wildtype and novel alleles in primary tumors and cell lines were calculated, defining a 2-fold difference as threshold for allelic shifts.
Korff et al. BMC Cancer 2008 8:329 doi:10.1186/1471-2407-8-329