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Table 2
Immunostaining of osteosarcoma-derived cell lines
					Cell line

	OSA-01		OSA-02		OSA-03		OSA-05		Saos-2
Antigen	%	IR	%	IR	%	IR	%	IR	%	IR

Desmin	+++	+	++	++	+++	++	++	+	++	+
S-100	+++	+	+++	+	++	+	+++	++	+++	+
Vimentin	++	+	++	+	+++	++	+++	+++	++	++
Nestin	++	++	++	++	+++	+++	-	-	+	+++
CD133	+++	++	+++	+++	+++	+++	+++	++	+++	+++

	%		%		%		%		%

Nestin/CD133 co-expression	++		++		+++		-		+

Expression of nestin, CD133 and other proteins was examined on cell cultures of osteosarcoma-derived cell lines using indirect immunofluorescence. With the exception of the nestin-negative OSA-05 cell line, all cell lines showed even immunoreactivity for the detected antigens. Percentage of positive cells in cell populations (%: -, 0%; +/-, <2 %; +, 2–10 %; ++, 11–50 %; +++, 51–100 %) and intensity of immunostaining (IR: -, none; +, weak; ++, medium; +++, strong) were evaluated. Saos-2 cell line was originally used as a control osteosarcoma cell line for the CD133 staining.
Veselska et al. BMC Cancer 2008 8:300 doi:10.1186/1471-2407-8-300

Table 2