Reduction of CDC2 mRNA and protein in SHG44 cell lines by recombinant C1 or C3. A, B, RT-PCR analysis: SHG44 cells were treated with recombinant C1 or C3, C4, blank retroviral vector (C0) and transfection reagent oligofectamine alone (X) for 48 h, β-actin served as an equal loading control. C, Western blot analysis, the membrane was stained with antibodies against CDC2 and GAPDH. The latter served as an equal loading control.
Chen et al. BMC Cancer 2008 8:29 doi:10.1186/1471-2407-8-29