Effects of NS-398 on expression of COX-1, COX-2 and PGE2. (A). COX-1 and COX-2 expression in MOR/P cells was detected by Western blotting after incubation for 24 h with NS-398 at concentration of 0–20 μM. (B). PGE2 production was measured by ELISA in A549 cell line after treatment with NS-398 2 μM (stripe bar) and 20 μM (solid bar) for 48 h, or control (open bar). (C). PGE2 production was measured by ELISA in three NSCLC cell lines after treatment with NS-398 20 μM (solid bar) for 24 h, or control (open bar). Each bar is the mean ± SEM of three determinations. ** P < 0.01.
Zhu et al. BMC Cancer 2008 8:218 doi:10.1186/1471-2407-8-218