Figure 5.

VA Qu Spez-treated DCs stimulate melanoma specific M77-80 CTL clone. The 4-day-old DCs (0.5 × 10⁶) from HLA-A2 donor were treated with medium alone (Control) or with 15 ng/ml TNF-α or with 15 μg/ml of VA Qu Spez for 48 hours. The DCs (10⁴/well/200 μl medium) were then cultured overnight with M77-80 CTLs (10⁵) in 96 well round-bottomed plates along with the MART-1 peptide (1 μM) and 25 IU/mL rh IL-2. The activation of M77-80 was analyzed by measuring TNFα (Panel A) and IFNγ (Panel B) in the cell free-supernatants by using cytokine bead array.