Solid tumor and cell line expression of keratins, TP53 and HER2. A-D) Immunohistochemistry on paraffin embedded TOV-1946 tumor tissue verifying Krt7, Krt20, Her2 and p53 expression respectively. E-H) Expression of the same four proteins was verified in the TOV-2223 tumoral tissue. Krt7 and Krt20 were used to distinguish between ovarian and gastro-intestinal tract tumors. Nuclei were counterstained with hematoxylin and all sections were observed by light microscopy at 400× magnification. I) In parallel, Her2, p53, Krt7, Krt18 and Krt8 expression was verified by western blot in cell lines. Krt7, Krt18 were used in order to confirm the epithelial character of cell lines. Actin was used as a loading control.
Ouellet et al. BMC Cancer 2008 8:152 doi:10.1186/1471-2407-8-152