Figure 5.

Western analysis indicates reduced TFIIS protein after TFIIS siRNA treatment. For the Western analysis, MCF7 cells were treated with vehicle, S-siRNA or TFIIS siRNA#2 as indicated. TFIIS and alpha-tubulin monoclonal antibodies were employed to detect their respective proteins. The ratio of TFIIS to alpha-tubulin as determined by imagequant (BioRad) is depicted by the bar-graph and listed as well above the columns. Cells were collected 48 hours after initial siRNA treatment.