Open Access Highly Accessed Research article

Growth of human gastric cancer cells in nude mice is delayed by a ketogenic diet supplemented with omega-3 fatty acids and medium-chain triglycerides

Christoph Otto1*, Ulrike Kaemmerer2, Bertram Illert3, Bettina Muehling1, Nadja Pfetzer2, Rainer Wittig4, Hans Ullrich Voelker5, Arnulf Thiede3 and Johannes F Coy4

Author Affiliations

1 Experimental Transplantation Immunology, Department of Surgery, University of Würzburg Hospital, Oberdürrbacher Str. 6, D-97080 Würzburg, Germany

2 Department of Obstetrics and Gynaecology, University of Würzburg Hospital, Josef-Schneider-Str. 4, D-97080 Würzburg, Germany

3 Department of Surgery, University of Würzburg Hospital, Oberdürrbacher Str. 6, D-97080 Würzburg, Germany

4 Tavartis GmbH, Kroetengasse 10, D-64853 Otzberg, Germany

5 Institute of Pathology, University of Würzburg, Josef-Schneider-Str. 2, D-97080 Würzburg, Germany

For all author emails, please log on.

BMC Cancer 2008, 8:122  doi:10.1186/1471-2407-8-122

Published: 30 April 2008

Additional files

Additional file 1:

Immunohistochemical analysis of representative tumours of the KD (animal 9) and SD (animal 16) groups. The carcinoma cells (pan cytokeratin-positive) located within the viable zone around the necrosis have proliferated (Ki-67 antigen-positive) and exhibit a glycolytic phenotype (TKTL1-positive). The expression of Glut-1 correlated with the strong glucose uptake and lactate production shown in Fig. 1 (Magnification: ×250).

Format: PDF Size: 382KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Influence of the ketogenic diet on vascularity in tumours of the human gastric adenocarcinoma cell line 23132/87. Representative tumour sections from the KD (animal 11) and SD (animal 13) groups are shown. Vessels were stained with the rat anti-mouse CD34 antibody RAM34 as described in Methods (Magnification: ×400).

Format: PDF Size: 362KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data