Research article

Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference

Chen Liu^{* 1} , Yaohui Chen^{* 2} , Xianjun Yu¹ , Chen Jin¹ , Jin Xu¹ , Jiang Long¹ , Quanxing Ni¹ , Deliang Fu¹ , Hong Jin² and Chen Bai²

¹Pancreatic Disease Institute, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China

²Department of Biochemistry, Fudan University, Shanghai 200433, PR China

author email corresponding author email* Contributed equally

BMC Cancer 2008, 8:121doi:10.1186/1471-2407-8-121

Published:	29 April 2008

Abstract

Background

Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation of tumor suppressor genes during tumorigenesis. Over-expression of MBD1 has been reported in human pancreatic carcinomas.

Methods

In this study, we established a MBD1-knock-down pancreatic cancer cell line (BxPC-3) using stable RNA interference, to compare the proteomic changes between control and MBD1-knock-down cells using two-dimensional gel electrophoresis and mass spectrometry.

Results

We identified five proteins that were up-regulated and nine proteins that were down-regulated. Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors.

Conclusion

Our data suggest that these differential proteins may be associated with the function of MBD1, and provide some insight into the functional mechanism of MBD1 in the development of pancreatic cancer.