Research article
Repeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins
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* Corresponding author: Ricardo Saban ricardo-saban@ouhsc.edu
1 Department of Physiology, The University Oklahoma Health Sciences Center, Oklahoma City, USA
2 Department of Anesthesiology, University of Texas Medical Branch, Galveston, USA
3 Department of Microbiology and Immunology The University Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
4 Department of Cell Biology, The University Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
5 Department of Urology, University of Iowa, UI Hospitals and Clinics, Iowa City, Iowa 52242-1089, USA
6 Department of Urology, New York University, School of Medicine, New York, NY 10016, USA
BMC Cancer 2007, 7:204 doi:10.1186/1471-2407-7-204
Published: 2 November 2007Additional files
Additional file 1:
Agarose gel electrophoresis of RNA samples. C = saline-treated and T=BCG-treated.
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Additional file 2:
Agarose gel electrophoresis of ds cDNA synthesis and Rsa I digestion. Lane 1-SMART-amplified C cDNA (driver); Lane 2-SMART-amplified T cDNA (tester); Lane 3-Rsa I digested C cDNA; and Lane 4-Rsa I digested T cDNA M = 1 kb DNA size markers.
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Additional file 3:
Table 1 Oligonucleotides used
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Additional file 4:
Agarose gel electrophoresis of primary and secondary PCR products. Lane M = 1 kb DNA size markers, Lane 1 = primary PCR of C subtracted cDNA; Lane 2 = primary PCR of T subtracted cDNA; Lane 3 = secondary PCR of C subtracted cDNA; Lane 4 = secondary PCR of T subtracted cDNA; Lane 5 = unsubtracted C cDNA; and Lane 6 = unsubtracted T cDNA.
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Additional file 5:
Table 2. QOCR Primers
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Additional file 6:
Differential screening of plate C-1 from C (driver) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 7:
Differential screening of plate C-2 from C (driver) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 8:
Differential screening of plate T-1 from T (tester) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 9:
Differential screening of plate T-2 from T (tester) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 10:
Differential screening of plate T-3 from T (tester) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 11:
Differential screening of plate T-4 from T (tester) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 12:
Differential screening of plate T-5 from T (tester) subtracted library was subjected to differential screening using driver-specific (A) and tester-specific (B) subtracted probes.
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Additional file 13:
Virtual Northern blot analysis of differential clones obtained from control bladder (C) subtracted library. A = Plate C-1 and B = Plate C-2
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Additional file 14:
Virtual Northern blot analysis of differential clones obtained from BCG-treated bladder (T) subtracted library. A = Plate T-1; B = Plate T-2; C = Plate T-3; D = Plate T-4; and E = Plate T-5.
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Additional file 15:
Table 3. Genes up-regulated by BCG
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Additional file 16:
Table 4. Genes down-regulated by BCG.
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Additional file 17:
Table 5. Ingenuity Summary BCG.
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Additional file 18:
Table 6. Ingenuity Summary Control.
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