Table 2 |
|
|
Antibody source and usage |
|
|
Primary antibodies |
Secondary antibodies |
|
|
|
|
Polyclonal goat anti-human REC8 (E-18):sc-15152 (Santa Cruz) |
Rabbit anti-goat-Cy3 (Sigma) |
|
Polyclonal rabbit anti-mouse REC8 antibody (602; kindly donated by Dr. C. Heyting, Wageningen, NL [23]) |
Goat anti-rabbit IgG-Texas red (Vector Laboratories Ltd., UK), |
|
CREST serum against human kinetochore proteins (Acris Antibodies, Hiddenhausen, Germany) |
Mouse anti-human IgG-FITC (Dr. MS Cragg) |
|
Polyclonal rabbit anti-MOS (C237: sc-86; Santa Cruz Biotech;Wembley, UK) |
Mouse anti-rabbit IgG-FITC (Sigma, Dorset, UK) |
|
Polyclonal rabbit anti-SCP3 antiserum (kindly donated by Dr. C. Heyting, Wageningen, NL [46]) |
Goat anti-rabbit IgG-FITC (Vector Laboratories Ltd., UK), |
|
|
|
|
Kalejs et al. BMC Cancer 2006 6:6 doi:10.1186/1471-2407-6-6 |
|
