Figure 4.

Schematic representation of methylation-specific PCR in CC. (A) PCR analysis of CC: M, DNA size marker; Neg, water; N and T, matched normal cervices and tumors, respectively. (B) Bar graph demonstrating the frequency of DNA methylation. Numbers on the y-axis are the percentage of positive methylation cases. Sample types are on the x-axis. WBC, normal cervix, Low-grade SIL, High-grade SIL, microinvasive cancer and squamous cell CCs number are 43, 25, 13, 11, 5, and 30, respectively. The methylation frequencies of each tissue type are represented by the height of each rectangular bar.