Figure 6.

Semi quantitative RT-PCR analysis of NCAM 140 kDa and 180 kDa RNA in parental UKF-NB-2, UKF-NB-3, UKF-NB-4, IMR-32 (Ctrl) versus vincristine (VCR) or doxorubicin resistant subpopulations (DOX). RNA were extracted, reverse-transcribed, and submitted to semiquantitative reverse transcription-PCR using gene specific primers as indicated in materials and methods. The internal control for the RT-PCR reaction was performed by running parallel reaction mixtures with the housekeeping gene GAPDH. The figure shows one representative from three separate experiments.