Table 3

Relative gene expression levels measured by real time kinetic PCR in Caco-2 and NHEK cells after NAC treatment as compared to untreated cells.

Caco-2

Gene

Common

Genbank

1h

12h

24h

48h


RT-PCR

Affymetrix

RT-PCR

Affymetrix

RT-PCR

Affymetrix

RT-PCR

Affymetrix


matrix metalloproteinase 9*

MMP9

J05070

na

nd

no change

up

na

up

na

na

aldo-keto reductase*

AKR1C3

D17793

na

nd

no change

up

up (2.8)

nd

na

na

aquaporin 3*

AQP3

N74607

no change

down

up (6.8)

up

up (5.0)

up

na

na

plasminogen activator, tissue*

PLAT

M15518

na

nd

no change

nd

no change

nd

na

na

HMG-box transcription factor 1*

HBP1

AF019214

no change

nd

up (2.6)

up

up (2.1)

nd

na

na

Cox-2*

PTGS2

U04636

no change

nd

no change

nd

down (2.2)

down

na

na

v-erb-b2*

ERBB3

M34309

na

nd

up (3.7)

up

no change

nd

na

na

proline-rich nuclear receptor coactivator 1*

PNRC1

U03105

na

nd

no change

up

up (2.4)

nd

na

na

inhibitor of differentiation*

ID1

AA457158

down (3.8)

down

na

nd

na

nd

na

nd

E-cadherin**

CDH1

Z13009

na

nd

no change

nd

up

nd

no change

na

hsp27**

HSPB1

U90906

na

nd

up

nd

up

nd

no change

na

p53**

TP53

U94788

na

nd

up

nd

no change

nd

no change

na

N-myc downstream regulated gene 1**

NDRG1

D87953

na

nd

up

nd

up

up

up

na

NHEK

30 min

3h

12h

24h

48h


Gene

Common

Genbank

RT-PCR

Affymetrix 1 h

RT-PCR

Affymetrix

RT-PCR

Affymetrix

RT-PCR

Affymetrix

RT-PCR

Affymetrix


matrix metalloproteinase 9*

MMP9

J05070

no change

nd

no change

nd

na

up

na

up

up (11.1)

nd

aldo-keto reductase*

AKR1C3

D17793

na

nd

down (4.0)

nd

na

nd

na

nd

down (5.3)

nd

aquaporin 3*

AQP3

N74607

no change

nd

no change

nd

na

nd

na

up

up (2.8)

nd

plasminogen activator, tissue*

PLAT

M15518

no change

nd

no change

nd

na

nd

na

down

no change

nd

HMG-box transcription factor 1*

HBP1

AF019214

down (6.3)

nd

down (4.0)

nd

na

up

na

nd

no change

nd

Cox-2*

PTGS2

U04636

down (7.7)

nd

down (6.3)

nd

na

nd

na

down

down (8.3)

nd

v-erb-b2*

ERBB3

M34309

na

nd

down (2.4)

nd

na

up

na

nd

down (4.5)

nd

proline-rich nuclear receptor coactivator 1*

PNRC1

U03105

na

nd

down (5.3)

nd

na

up

na

nd

no change

nd

inhibitor of differentiation*

ID1

AA457158

down (8.3)

down

na

nd

na

nd

na

nd

na

nd

E-cadherin**

CDH1

Z13009

na

nd

na

nd

no change

nd

up

nd

up

nd

hsp27**

HSPB1

U90906

na

nd

na

nd

up

nd

up

nd

up

nd

p53**

TP53

U94788

na

nd

na

nd

no change

nd

up

nd

up

nd

N-myc downstream regulated gene 1**

NDRG1

D87953

na

nd

na

nd

no change

nd

no change

nd

no change

nd


Significant fold change: <0.5 for down regulated and >2.0 for up regulated genes, respectively. na = not analysed; nd = no data. * Taqman RT-PCR was done on these genes using Assays-on-demand (Applied Biosystems). GAPDH was used as a reference gene. **SYBR green RT-PCR was done on these genes using in house designed primers. Transferrin receptor was used as a reference gene.

Gustafsson et al. BMC Cancer 2005 5:75   doi:10.1186/1471-2407-5-75

Open Data