Table 3 |
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|
Effects of in vitro transcribed ENO1 mRNA constructs on cell growth after transfection into neuroblastoma (SK-N-AS), 293 and K562 cell lines. |
||||||||||
|
cell line |
SK-N-AS |
293 |
K562 |
|||||||
|
|
||||||||||
|
mRNA |
day |
% decrease in number of cells +/-SD |
no of exp |
p-value test |
% decrease in number of cells +/-SD |
no of exp |
p-value test |
% decrease in number of cells +/-SD |
no of exp |
p-value test |
|
|
||||||||||
|
1.5 μg EGFP |
- |
4 × 8 |
- |
3 × 8 |
- |
2 × 4 |
||||
|
1.5 μg ENO1 |
1 |
58 +/- 12 |
4 × 8 |
< 10-5 |
52 +/- 11 |
3 × 8 |
< 10-3 |
-11 +/- 19 |
2 × 4 |
> 0.05 |
|
2 |
81 +/- 11 |
< 10-9 |
85 +/- 8 |
< 10-5 |
13 +/- 34 |
> 0.05 |
||||
|
3 |
79 +/- 12 |
< 10-9 |
89 +/- 16 |
< 10-5 |
9 +/- 11 |
> 0.05 |
||||
|
4 |
76 +/- 17 |
< 10-9 |
87 +/- 20 |
< 10-5 |
10 +/- 9 |
0.03 |
||||
|
0.3 μg ENO1 |
1 |
39 +/- 25 |
3 × 8 |
0.02 |
38 +/- 27 |
2 × 8 |
< 10-4 |
-14 +/- 14 |
1 × 4 |
0.04 |
|
2 |
63 +/- 15 |
< 10-5 |
64 +/- 36 |
< 10-4 |
6 +/- 11 |
> 0.05 |
||||
|
3 |
60 +/- 11 |
< 10-5 |
60 +/- 54 |
< 10-4 |
4 +/- 10 |
> 0.05 |
||||
|
4 |
64 +/- 13 |
< 10-3 |
59 +/- 52 |
< 10-5 |
-3 +/- 9 |
> 0.05 |
||||
|
1.5 μg ENO1-mut |
1 |
65 +/- 26 |
2 × 8 |
0.007 |
44 +/- 9 |
1 × 8 |
< 10-3 |
-6 +/- 14 |
2 × 4 |
> 0.05 |
|
2 |
84 +/- 8 |
< 10-3 |
77 +/- 4 |
< 10-6 |
20 +/- 8 |
0.004 |
||||
|
3 |
84 +/- 8 |
< 10-4 |
73 +/- 6 |
< 10-4 |
16 +/- 13 |
0.03 |
||||
|
4 |
84 +/- 7 |
< 10-3 |
63 +/- 6 |
< 10-6 |
5 +/- 11 |
> 0.05 |
||||
|
0.3 μg ENO1-mut |
1 |
66 +/- 8 |
1 × 8 |
< 10-3 |
20 +/- 13 |
1 × 8 |
0.02 |
-18 +/- 13 |
1 × 4 |
0.01 |
|
2 |
82 +/- 4 |
< 10-6 |
50 +/- 7 |
< 10-4 |
8 +/- 12 |
> 0.05 |
||||
|
3 |
70 +/- 7 |
< 10-4 |
28 +/- 15 |
0.007 |
9 +/- 13 |
> 0.05 |
||||
|
4 |
72 +/- 6 |
< 10-4 |
29 +/- 6 |
< 10-4 |
-1 +/- 11 |
> 0.05 |
||||
|
1.5 μg MBP-1 |
1 |
45 +/- 21 |
4 × 8 |
< 10-3 |
41 +/- 8 |
3 × 8 |
0.005 |
-9 +/- 16 |
2 × 4 |
> 0.05 |
|
2 |
67 +/- 13 |
< 10-7 |
75 +/- 10 |
< 10-4 |
10 +/- 12 |
> 0.05 |
||||
|
3 |
68 +/- 15 |
< 10-6 |
82 +/- 25 |
< 10-4 |
1 +/- 8 |
> 0.05 |
||||
|
4 |
64 +/- 20 |
< 10-7 |
76 +/- 31 |
< 10-4 |
5 +/- 9 |
> 0.05 |
||||
|
0.3 μg MBP-1 |
1 |
25 +/- 18 |
3 × 8 |
> 0.05 |
50 +/- 38 |
2 × 8 |
0.02 |
-14 +/- 14 |
1 × 4 |
0.04 |
|
2 |
47 +/- 18 |
0.001 |
56 +/- 18 |
< 10-5 |
4 +/- 18 |
> 0.05 |
||||
|
3 |
38 +/- 7 |
0.001 |
44 +/- 46 |
< 10-3 |
-11 +/- 17 |
> 0.05 |
||||
|
4 |
37 +/- 19 |
0.006 |
42 +/- 34 |
< 10-4 |
-2 +/- 8 |
> 0.05 |
||||
|
|
||||||||||
|
Ejeskär et al. BMC Cancer 2005 5:161 doi:10.1186/1471-2407-5-161 |
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