Table 3

Effects of in vitro transcribed ENO1 mRNA constructs on cell growth after transfection into neuroblastoma (SK-N-AS), 293 and K562 cell lines.
cell line
 SK-N-AS
 293
 K562
mRNA
 day
 % decrease in
number of
cells +/-SD
 no of exp
 p-value test
 % decrease in
number of
cells +/-SD
 no of exp
 p-value test
 % decrease in
number of
cells +/-SD
 no of exp
 p-value test
1.5 μg EGFP
-
 4 × 8
-
 3 × 8
-
 2 × 4
1.5 μg ENO1
 1
 58 +/- 12
 4 × 8
 < 10-5
 52 +/- 11
 3 × 8
 < 10-3
 -11 +/- 19
 2 × 4
 > 0.05

2
 81 +/- 11
< 10-9
 85 +/- 8
< 10-5
 13 +/- 34
> 0.05

3
 79 +/- 12
< 10-9
 89 +/- 16
< 10-5
 9 +/- 11
> 0.05

4
 76 +/- 17
< 10-9
 87 +/- 20
< 10-5
 10 +/- 9
0.03
0.3 μg ENO1
 1
 39 +/- 25
 3 × 8
 0.02
 38 +/- 27
 2 × 8
 < 10-4
 -14 +/- 14
 1 × 4
 0.04

2
 63 +/- 15
< 10-5
 64 +/- 36
< 10-4
 6 +/- 11
> 0.05

3
 60 +/- 11
< 10-5
 60 +/- 54
< 10-4
 4 +/- 10
> 0.05

4
 64 +/- 13
< 10-3
 59 +/- 52
< 10-5
 -3 +/- 9
> 0.05
1.5 μg ENO1-mut
 1
 65 +/- 26
 2 × 8
 0.007
 44 +/- 9
 1 × 8
 < 10-3
 -6 +/- 14
 2 × 4
 > 0.05

2
 84 +/- 8
< 10-3
 77 +/- 4
< 10-6
 20 +/- 8
0.004

3
 84 +/- 8
< 10-4
 73 +/- 6
< 10-4
 16 +/- 13
0.03

4
 84 +/- 7
< 10-3
 63 +/- 6
< 10-6
 5 +/- 11
> 0.05
0.3 μg ENO1-mut
 1
 66 +/- 8
 1 × 8
 < 10-3
 20 +/- 13
 1 × 8
 0.02
 -18 +/- 13
 1 × 4
 0.01

2
 82 +/- 4
< 10-6
 50 +/- 7
< 10-4
 8 +/- 12
> 0.05

3
 70 +/- 7
< 10-4
 28 +/- 15
0.007
 9 +/- 13
> 0.05

4
 72 +/- 6
< 10-4
 29 +/- 6
< 10-4
 -1 +/- 11
> 0.05
1.5 μg MBP-1
 1
 45 +/- 21
 4 × 8
 < 10-3
 41 +/- 8
 3 × 8
 0.005
 -9 +/- 16
 2 × 4
 > 0.05

2
 67 +/- 13
< 10-7
 75 +/- 10
< 10-4
 10 +/- 12
> 0.05

3
 68 +/- 15
< 10-6
 82 +/- 25
< 10-4
 1 +/- 8
> 0.05

4
 64 +/- 20
< 10-7
 76 +/- 31
< 10-4
 5 +/- 9
> 0.05
0.3 μg MBP-1
 1
 25 +/- 18
 3 × 8
 > 0.05
 50 +/- 38
 2 × 8
 0.02
 -14 +/- 14
 1 × 4
 0.04

2
 47 +/- 18
0.001
 56 +/- 18
< 10-5
 4 +/- 18
> 0.05

3
 38 +/- 7
0.001
 44 +/- 46
< 10-3
 -11 +/- 17
> 0.05

4
 37 +/- 19
0.006
 42 +/- 34
< 10-4
 -2 +/- 8
> 0.05

Ejeskär et al. BMC Cancer 2005 5:161   doi:10.1186/1471-2407-5-161