Figure 3.

Immunohistochemical localization of phospho-Akt and total Akt in human squamous cell carcinoma. Serial section from human squamous cell carcinoma was stained with antibodies specific for phospho-Akt (ser473) (A), or an antibody that recognizes both phosphorylated and non-phosphorylated forms of Akt (B). Localization of phospho-Akt to the plasma membrane of a subset of tumor cells is apparent. Incubation without primary antibody (C) was used as a negative control.