Figure 1.

Northern Blot Analysis of DARC-A549 transfectants. 10 μg of total RNA from each cell clone was subjected to northern blot transfer. Expression levels of mRNA for each probe was densitometrically analyzed and compared to the signal obtained for the 28S rRNA. We observed DARC expression only in the A549 cells that had been transfected with a DARC-containing plasmid while the pTARGET control plasmid transfectanted appeared negative for DARC expression.