Email updates

Keep up to date with the latest news and content from BMC Cancer and BioMed Central.

Open Access Highly Accessed Research article

The histone deacetylase inhibitor Trichostatin A modulates CD4+ T cell responses

José Manuel Afonso Moreira12*, Peter Scheipers13 and Poul Sørensen14

Author Affiliations

1 Department of Biology, Active Biotech Research AB, P.O. Box 724, SE-22007 Lund, Sweden

2 Institute of Cancer Biology and Danish Centre for Translational Breast Cancer Research, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen Ø, Denmark

3 Department of Cellular and Molecular Biology, Active Biotech Research AB, Scheelev. 22, P.O. Box 724, SE-22007 Lund, Sweden

4 Micromet AG, Staffelseestrasse 2, 81477 Munich, Germany

For all author emails, please log on.

BMC Cancer 2003, 3:30  doi:10.1186/1471-2407-3-30

Published: 9 November 2003

Abstract

Background

Histone deacetylase inhibitors (HDACIs) induce hyperacetylation of core histones modulating chromatin structure and affecting gene expression. These compounds are also able to induce growth arrest, cell differentiation, and apoptotic cell death of tumor cells in vitro as well as in vivo. Even though several genes modulated by HDAC inhibition have been identified, those genes clearly responsible for the biological effects of these drugs have remained elusive. We investigated the pharmacological effect of the HDACI and potential anti-cancer agent Trichostatin A (TSA) on primary T cells.

Methods

To ascertain the effect of TSA on resting and activated T cells we used a model system where an enriched cell population consisting of primary T-cells was stimulated in vitro with immobilized anti-CD3/anti-CD28 antibodies whilst exposed to pharmacological concentrations of Trichostatin A.

Results

We found that this drug causes a rapid decline in cytokine expression, accumulation of cells in the G1 phase of the cell cycle, and induces apoptotic cell death. The mitochondrial respiratory chain (MRC) plays a critical role in the apoptotic response to TSA, as dissipation of mitochondrial membrane potential and reactive oxygen species (ROS) scavengers block TSA-induced T-cell death. Treatment of T cells with TSA results in the altered expression of a subset of genes involved in T cell responses, as assessed by microarray gene expression profiling. We also observed up- as well as down-regulation of various costimulatory/adhesion molecules, such as CD28 and CD154, important for T-cell function.

Conclusions

Taken together, our findings indicate that HDAC inhibitors have an immunomodulatory potential that may contribute to the potency and specificity of these antineoplastic compounds and might be useful in the treatment of autoimmune disorders.