Expression of phospho and total JAK2 and STAT3 in control, paclitaxel and paclitaxel plus CYT387-treated HEY cells. (A) Expression and immunolocalisation of phospho (P)-JAK2 (Tyr-1007/1008) and phospho (P)-STAT3 (Tyr-705) in control, paclitaxel, CYT387 and combination of both treatments in HEY cell line was evaluated by immunofluorescence. Three independent experiments were performed in triplicate. The mean fluorescence intensity was quantified using Cell-R software. Significant variations between the groups are indicated by *P<0.05, *** P < 0.001. (B) The expression of total (T)-JAK2 and total (T)-STAT3 was evaluated and quantified as described in Figure 6A. Magnification 200x; scale bar = 10 μM.
Abubaker et al. BMC Cancer 2014 14:317 doi:10.1186/1471-2407-14-317