Increased expressions of CSC and embryonic stem cell markers in ascites- derived tumor cells in response to paclitaxel. Expression and localisation of EpCAM, CD117, Oct4 and NANOG in ascites-derived tumor cells in response to paclitaxel treatment was evaluated by immunofluorescence as described in Figure 1. Images are representative of three independent experiments from three independent patient ascites samples. The mean fluorescence intensity of CSC markers CD117, EpCAM and the embryonic stem cell markers NANOG and Oct4 expression in ascites-derived tumor cells was quantified using Cell-R software. Significant variations between the groups are indicated by *P < 0.05. Magnification 200×; scale bar = 10 μM.
Abubaker et al. BMC Cancer 2014 14:317 doi:10.1186/1471-2407-14-317