Increased expression of β-tubulin III and ERCC1 in ascites-derived tumor cells in response to paclitaxel. Expression and immunolocalisation of β-tubulin III and ERCC1 in ascites-derived tumor cells was evaluated by immunofluorescence using mouse monoclonal (green) and rabbit polyclonal (red) antibodies as described in the Methods. Cellular staining was visualized using secondary Alexa 488 (green) and Alexa 590 (red) fluorescent labelled antibodies while nuclear staining was visualized using DAPI (blue) staining. Images are representative of three independent experiments from three independent patient samples. The mean fluorescence intensity of β-tubulin III and ERCC1 was quantified using Cell-R software. Significant variations between the groups are indicated by *P < 0.05. Magnification 200×; scale bar = 10 μM.
Abubaker et al. BMC Cancer 2014 14:317 doi:10.1186/1471-2407-14-317