Simultaneous expression of flotillin-1, flotillin-2, stomatin and caveolin-1 in non-small cell lung cancer and soft tissue sarcomas
1 Laboratory for Cellular and Viral Oncogene Regulation, Carcinogenesis Research Institute, N. N. Blokhin Russian Cancer Research Center RAMS, 24, Kashirskoye sh., Moscow 115478, Russia
2 Thoraco-Abdominal Oncology Department, Clinical Oncology Research Institute, N. N. Blokhin Russian Cancer Research Center RAMS, 24, Kashirskoye sh., Moscow 115478, Russia
3 Human Tumor Pathologic Anatomy Department, Clinical Oncology Research Institute, N. N. Blokhin Russian Cancer Research Center RAMS, 24, Kashirskoye sh., Moscow 115478, Russia
BMC Cancer 2014, 14:100 doi:10.1186/1471-2407-14-100Published: 17 February 2014
At the present time, there is a lack of data about the involvement of flotillins and stomatin in the development of non-small cell lung cancer (NSCLC) and soft tissue sarcomas (STS). Moreover, changes in expression of members of different families of the microdomain-forming proteins (caveolins and SPFH-domain containing family) are usually investigated independently of each other. In this study we performed a combined analysis of flotillins, stomatin, and caveolin-1 expression in these pathologies and evaluated correlations between generated data and clinicopathological characteristics of the specimens.
The protein and mRNA expression was analyzed by Western blotting and real-time PCR, respectively, in tissue specimens of patients undergoing surgery for non-small cell lung cancer and soft tissue sarcomas. Association between expression of studied proteins and patient clinicopathological characteristics or outcome was evaluated.
Stomatin protein expression was down-regulated in 80% of NSCLC samples and this decrease significantly associated with presence of lymph node metastases. Flotillin-2 protein expression was up-regulated in the majority of NSCLC samples whereas caveolin-1α expression was decreased. We revealed a strong correlation between STOM and FLOT-1 mRNA expression in both pathologies, although the gene expression changes were diverse.
Our data demonstrate for the first time that expression of stomatin, a poorly studied microdomain-forming protein, significantly changes in human tumors, thus pointing to its importance in the progression of NSCLC. We also suggest the existence of some relationship between the expression of these proteins.