In vivo recruitment of MBP-1 and HDAC1 proteins to ERBB2 and c-MYC promoters. (A) Immunoblot analysis of SKBr3 cells transfected with pFlag-MBP-1 or mock-transfected using anti-Flag, anti-HDAC1 and anti-beta-actin antibodies. (B) MBP-1 and HDAC1 occupancy at ERBB2 and c-MYC promoter DNA of input and immunoprecipitated chromatin samples was amplified using primers directed to ERBB2 promoter (ERP2/3), to c-MYC. P2 promoter (MP3/4) and primers directed to an unrelated region of the c-MYC gene (MD). (C, D) Quantification by real-time PCR of chromatin immunoprecipitated with anti-HDAC1 and anti-AcH4 antibodies. The amount of immunoprecipitated DNA was calculated relative to that present in total input chromatin (% input). Each data point is the average of triplicates from three independent ChIP experiments ± SD, p value (* P<0.01) indicate statistical significance.
Contino et al. BMC Cancer 2013 13:81 doi:10.1186/1471-2407-13-81