Open Access Research article

Negative transcriptional control of ERBB2 gene by MBP-1 and HDAC1: diagnostic implications in breast cancer

Flavia Contino1, Claudia Mazzarella1, Arianna Ferro1, Mariavera Lo Presti13, Elena Roz3, Carmelo Lupo3, Giovanni Perconti2, Agata Giallongo2* and Salvatore Feo12*

Author Affiliations

1 Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari, Università di Palermo, Viale delle Scienze, Ed. 16, Palermo I-90128, Italy

2 Istituto di Biomedicina e Immunologia Molecolare, CNR, Via Ugo La Malfa, 153, Palermo I-90146, Italy

3 Unità di Anatomia Patologica, Dipartimento Oncologico di III livello La Maddalena, Palermo, Italy

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BMC Cancer 2013, 13:81  doi:10.1186/1471-2407-13-81

Published: 19 February 2013

Additional files

Additional file 1: Table S1:

List of gene-specific oligonucleotides used in this study.

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Additional file 2: Figure S1:

Immunofluorescence microscopy images showing intracellular localization of endogenous ErbB2 and either ectopically expressed MBP-1 or GFP protein. Human SKBr3 cancer cells transiently expressing either Flag-MBP-1 or GFP protein (upper and lower panels, respectively) were fixed, permeabilized and double-stained with anti-ErBB2 and anti-Flag antibody or single-stained with anti-ErbB2, as indicated. Nuclei were stained with DAPI. Spatial distribution was visualized by light microscopy as described in Materials and Methods. The colour merged images show the loss of ErbB2 membrane staining in MBP-1-expressing cells (upper panel). Scale bar, 25 um.

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Additional file 3: Figure S2:

Nucleotide sequence of the human ERBB2 promoter and upstream regions. The nucleotide sequence is numbered with the major transcription start site designated as + 1 (according to NCBI RefSeq: NG_007503.1). Positions of relevant restriction sites are indicated and A/T-rich elements are boxed. Arrows indicate the position of oligonucleotides used for the construction of the ERBB2-luciferase reporter plasmids and for ChIP-qPCR assays (see Additional file 1).

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