Open Access Research article

Methyl jasmonate abolishes the migration, invasion and angiogenesis of gastric cancer cells through down-regulation of matrix metalloproteinase 14

Liduan Zheng12, Dan Li3, Xuan Xiang3, Ling Tong1, Meng Qi3, Jiarui Pu3, Kai Huang24 and Qiangsong Tong23*

Author Affiliations

1 Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People’s Republic of China

2 Clinical Center of Human Genomic Research, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People’s Republic of China

3 Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People’s Republic of China

4 Department of Cardiology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, People’s Republic of China

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BMC Cancer 2013, 13:74  doi:10.1186/1471-2407-13-74

Published: 10 February 2013

Additional files

Additional file 1: Table S1:

Oligonucleotide sets used for constructs and small interfering RNAs.

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Additional file 2: Table S2:

Primer sets used for qRT-PCR.

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Additional file 3: Figure S1:

Time-course effects of sub-cytotoxic MJ on the viability of gastric cancer cells. Human gastric cancer SGC-7901 and MKN-45 cells were incubated with sub-cytotoxic (0.05, 0.1 and 0.2 mM) MJ for 6, 12 and 24 hrs. MTT colorimetric assay indicated that sub-cytotoxic MJ did not affect the viabilities of gastric cancer cells, when compared to those treated by solvent (mock).

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Additional file 4: Figure S2:

Sub-cytotoxic MJ did not affect the proliferation of human endothelial cells. Human endothelial HUVEC cells were incubated with sub-cytotoxic (0.05, 0.1 and 0.2 mM) MJ for 24 hrs. EdU incorporation assay indicated that sub-cytotoxic MJ did not affect the proliferation of HUVEC cells, when compared to those treated by solvent (mock).

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Additional file 5: Figure S3:

Transfection efficiency assay. Confluent monolayers of gastric cancer SGC-7901 and MKN-45 cells were transfected with the enhanced green fluorescent protein (EGFP) reporter vector pEGFP-N1. Seventy-two hrs post-transfection, EGFP expressed within the cytoplasm of cancer cells, with the transfection efficiency around 60%.

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