Figure 5.

Effect of Rac3 depletion on cell signaling. Cells were treated with 10 nM siRNA (control or Rac3). After 48 h of treatment, (A) total proteins were extracted and ERK-1, ERK-2 and their respective phosphorylated forms detected by western blot. (B) Cytoplasmic and nuclear proteins were extracted, and expression of NF-κB subunits detected by western blot. GAPDH is a control for cytoplasmic protein extract and H3 is a control for nuclear extract (C) Cytoplasmic and nuclear proteins were extracted from MDA-MB-231, and NF-κB p65 and p50 subunits detected by western blot. GAPDH is a control for cytoplasmic protein extract and histone H3 is a control for nuclear extract. (D) Total proteins were extracted from MDA-MB-231 and proteins of NF-κB activation pathways were detected by western blot. (E) Action of siRNA anti-Rac3 on cytokine secretion by MDA-MB-231 was detected by cytokine array. Mean ± S.E. N=3 independent experiments. * p<0.05; *** P<0.001.

Gest et al. BMC Cancer 2013 13:63   doi:10.1186/1471-2407-13-63
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