SPARC mRNA expression by qRT-PCR in cell subsets after magnetic separation tecnology. a. Distribution of SPARC expression in PBMCs of 4 HD and 4 CML patients at D and after 3 M of IM therapy calculated as percentage of expressing SPARC granulocytes, monocytes and lymphocytes in respect to the total number of separated expressing SPARC cells. Data are expressed as means ± S.D. b. SPARC mRNA levels in cell subsets of CML patients at D and after 3 M of therapy compared with the same cell populations of HC (calculated value of 2^-ΔΔCt in HD’ subsets was 1). Data are expressed as means ± S.D.
Giallongo et al. BMC Cancer 2013 13:60 doi:10.1186/1471-2407-13-60