Expression of LGR-5 in histological sections of rat colon as assessed by immunofluorescence technique. Panel A: a single stained cell (white arrow) at the lower compartment of the crypt in normal mucosa. Panel B: multiple positive cells at the base of normal crypts. Panel C: LGR-5 over-expression in a preneoplastic lesion MDF. Panel D: Magnification of the white inset in panel C showing: LGR-5 expressing cells (yellow arrow), cells expressing β-catenin in the nucleus (green arrow) and a cell co-expressing LGR-5 and nuclear β-catenin (white arrow). Original magnification 400x.
Femia et al. BMC Cancer 2013 13:48 doi:10.1186/1471-2407-13-48