Open Access Highly Accessed Research article

Molecular fingerprinting reflects different histotypes and brain region in low grade gliomas

Samantha Mascelli1*, Annalisa Barla2, Alessandro Raso1, Sofia Mosci2, Paolo Nozza3, Roberto Biassoni4, Giovanni Morana5, Martin Huber6, Cristian Mircean6, Daniel Fasulo7, Karin Noy7, Gayle Wittemberg7, Sara Pignatelli8, Gianluca Piatelli1, Armando Cama1, Maria Luisa Garré8, Valeria Capra1 and Alessandro Verri2

Author Affiliations

1 Neurosurgery Unit, Istituto Giannina Gaslini, via G. Gaslini 5, 16147, Genoa, Italy

2 DISI - Department of Computer Science, Università degli Studi di Genova, Via Dodecaneso, 35-16146, Genoa, Italy

3 Pathology Unit, Istituto Giannina Gaslini, via G. Gaslini 5, 16147, Genoa, Italy

4 Molecular Medicine Unit, Istituto Giannina Gaslini, via G. Gaslini 5, 16147, Genoa, Italy

5 Neuroradiology Unit, Istituto Giannina Gaslini, via G. Gaslini 5, 16147, Genoa, Italy

6 Siemens AG, Corporate Technology, Freyeslebenstr. 1, 91058, Erlangen, Germany

7 SCR - Siemens Corporate Research, Princeton, NJ, USA

8 Neuro-oncology Unit, Istituto Giannina Gaslini, via G. Gaslini 5, 16147, Genoa, Italy

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BMC Cancer 2013, 13:387  doi:10.1186/1471-2407-13-387

Published: 15 August 2013

Additional files

Additional file 1:

Workflow for high-throughput gene-expression studies. Description and discussion, including references, of the pipeline used for gene-expression studies.

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Additional file 2:

qPCR systems. Primers sequences and the amplification conditions of the 15 selected genes validated by qPCR, including 3 genes used as the endogenous control for each tumour specimen.

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Additional file 3:

Selected probe-sets by l1l2 for LGG related to the site of lesion. List of 331 probe-sets selected by the l1l2 procedure for LGG related to the site of lesion (infratentorial versus supratentorial). For each probe-set we report the corresponding Gene ID and its frequency score (f.s.).

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Additional file 4:

Microarray-based differences of LGG related to site of lesion. A brief comment of each locus, listing the main protein functions and including references, for the 15 selected genes that significantly discriminate infratentorial versus supratentorial LGGs.

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Additional file 5:

Pilocytic Astrocytomas - infratentorial vs supratentorial, selected probe-sets by the l1l2 for PA related to the site of lesion. List of 136 probe-sets selected by l1l2 for infratentorial vs supratentorial PAs. For each probe-set we report the Gene ID and its frequency score (f.s.). Genes reported in bold are those specific to PA histotype as a group.

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Additional file 6:

Supratentorial tumours: mixed glial-neuronal tumours vs. PAs, selected probe-sets by the l1l2. List of 103 probe-sets selected by the l1l2 procedure. For each probe-set we report the corresponding Gene ID and its frequency score (f.s.).

Format: XLS Size: 24KB Download file

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