Soft agar colony formation assay. Anchorage-independent growth in soft agar was examined under different concentrations of imatinib mesylate, and numbers of colonies were counted at day 7. (A) Numbers of anchorage-independent colonies of HS-Sch-2 cells growing in the soft agar were scored using phase contrast microscopy. (B) Imatinib mesylate at 5 μM significantly decreased the number of colonies of HS-Sch-2 and FMS-1 cells, but had no effect on NMS-2PC cells. Data are the means ± SEM (n = 6). *P < 0.01, compared with the control group without imatinib mesylate (by Student’s t-test).
Ohishi et al. BMC Cancer 2013 13:224 doi:10.1186/1471-2407-13-224