Expression of PDGFR proteins and mRNAs of in six MPNST cell lines analyzed by western blotting (A) and real-time RT-PCR (B). The mRNA expression levels in each cell line were compared with the levels in FU-SFT8611 cells. PDGFR-β protein and mRNA were expressed in all MPNST cell lines. The expression of PDGFR-α protein was observed in all but HS-Sch-2 cells. The PDGFR-α mRNA level in NMS-2 and NMS-2PC cells was higher than in the other four cell lines. Data in (B) is the mean ± SEM (n = 3). Similar results were obtained in three independent experiments. *P < 0.01, compared with the level in FU-SFT8611 cells (by Student’s t-test).
Ohishi et al. BMC Cancer 2013 13:224 doi:10.1186/1471-2407-13-224