In vitro effects of TNKS knockdown and rescue of Wnt pathway inhibition. (A) Protein expression levels of axin 1 following transient TNKS knockdown are shown by immunoblot analysis in ED1. (B) Protein expression levels of axin 1 following transient TNKS knockdown are shown by immunoblot analysis in Hop62. (C) Expression of activated β-catenin and total β-catenin in ED1 cells following stable infection with constitutively active β-catenin or empty vector. (D) Growth inhibition of ED1 cells infected as in C by TNKS inhibitors for 3 days at 10 μM is shown by luminescent cell viability assay. Error bars represent SD of three experiments in triplicate. (* p ≤ 0.05, ** p ≤ 0.01).
Busch et al. BMC Cancer 2013 13:211 doi:10.1186/1471-2407-13-211