Open Access Highly Accessed Research article

Evidence for tankyrases as antineoplastic targets in lung cancer

Alexander M Busch1, Kevin C Johnson1, Radu V Stan2347, Aarti Sanglikar5, Yashi Ahmed67, Ethan Dmitrovsky178 and Sarah J Freemantle1*

Author Affiliations

1 From the Department of Pharmacology and Toxicology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

2 Heart and Vascular Research Center, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

3 Department of Pathology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

4 Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

5 Center for Comparative Medicine and Research, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

6 Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

7 Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

8 Department of Medicine, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA

For all author emails, please log on.

BMC Cancer 2013, 13:211  doi:10.1186/1471-2407-13-211

Published: 28 April 2013

Additional files

Additional file 1: Figure S1:

Table containing all antibodies and dilutions, sequences for primers used to measure gene expression levels by qPCR, and siRNA and shRNA sequences used to knock-down TNKS expression levels.

Format: PDF Size: 278KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2: Figure S2:

Table reporting statistical analysis of main body figure 2 panels A, B, and C. Percent growth at each drug dosage was compared to vehicle control via ANOVA with Dunnett’s multiple comparisons post-test.

Format: PDF Size: 105KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Figure S3:

Figure showing washout studies for ED1 and A549 lung cancer cell lines treated with TNKS inhibitors.

Format: PDF Size: 276KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4: Figure S4:

In vivo syngeneic lung cancer tumor formation from injection of FVB mice with ED1 cells transduced with dual TNKS knockdown or dual control.

Format: PDF Size: 95KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data