Analysis of MAGEA and NY-ESO-1 expression. RT-PCR analysis of cells treated for 5 days with the indicated concentration of 5-AzaC, TSA or both compounds. DMSO was used as a mock control. Cells were lysed and mRNA was isolated and transcribed into cDNA. β-actin served as internal standard.
Schirmer et al. BMC Cancer 2013 13:156 doi:10.1186/1471-2407-13-156